Table 1.
mPepT1 | Forward primer | 5′-CCACGGCCATTTACCATACG-3′ |
Reverse primer | 5′-TGCGATCAGAGCTCCAAGAA-3′ | |
Probe | 5′-/56-FAM/-CGTTGCCCTCTGCTACCTGACTCCA-/36-TAMSp/-3′ | |
Standard | 5′-GACAATCTCTCCACGGCCATTTACCATACGTTCGTTG CCCTCTGCTACCTGACTCCAATTCTTGGAGCTCTGATCGCAGACTCGTGGC-3′ |
|
mPhT2 | Forward primer | 5′-TGCAGAGGCACGGACTAGATAC-3′ |
Reverse primer | 5′-GGGTGTGATGAACGTAGAAATCAA-3′ | |
Probe | 5′-/56-FAM/-TCTCGGTCTTCTACCTCTCCATCAATG CA-/36-TAMSp/-3′ |
|
Standard | 5′-AAGAGGAACATGCAGAGGCACGGACTAGATACTTCTC GGTCTTCTACCTCTCCATCAATGCAGGGAGCTTGATTTCTACGTTCATCACACC CATGCTAAG-3′ |
|
mPhT1 | Forward primer | 5′-GCTGCCACCTGCATTACTACTTC-3′ |
Reverse primer | 5′-CGTACTTCACAGACACAATGAGGAA-3′ | |
Probe | 5′-/56-FAM/-CTGGCCGCCATCCAGGGAGC-/36-TAMSp/-3′ | |
Standard | 5′-AACATCAACAGCTGCCACCTGCATTACTACTTCTTC CTGCTGGCCGCCATCCAGGGAGCTACGCTGCTGCTCTTCCTCATTGTGTCTGT GAAGTACGACCGCCAGCG-3′ |
|
mPhT2 | Forward primer | 5′-GCTGAAGCTTGCGTTCCAA-3′ |
Reverse primer | 5′-AACAGGTGGGCACTTTCAGAGT-3′ | |
Probe | 5′-/56-FAM/-CTGCTGTCCCTGCCGGAAGTCCT-/36-TAMSp/-3′ | |
Standard | 5′-TGTCATCTATGCTGAAGCTTGCGTTCCAAAACTGCTGTCCC TGCCGGAAGTCCTCTTCCAGGGACTCTGAAAGTGCCCACCTGTTGCCTGACC AGA-3′ |
|
mGAPDH | Forward primer | 5′-GAGACAGCCGCATCTTCTTGT-3′ |
Reverse primer | 5′-CACACCGACCTTCACCATTTT-3′ | |
Probe | 5′-/56-JOE/-CAGTGCCAGCCTCGTCCCGTAGA-/36-TAMSp/-3′ | |
Standard | 5′-TCCCTGTTCCAGAGACAGCCGCATCTTCTTGTGCAGTGCCA GCCTCGTCCCGTAGACAAAATGGTGAAGGTCGGTGTGAACGGATTTG-3′ |
|
rPhT1 | Forward primer | 5′-GGTGTGGCCACGGTCAA-3′ |
Reverse primer | 5′-GGCCTCTGGACCTCGATCTT-3′ | |
rGAPDH | Forward primer | 5′-TGGCCTCCAAGGAGTAAGAAAC-3′ |
Reverse primer | 5′-GGCCTCTCTCTTGCTCTCAGTATC-3′ |