Table 2. BLAST search results using HS4 and HS5 ORFs from the group 1 ICE in metagenomic datasets.
| Hotspot | ORF | Length (bp) | Function | Metagenomic project | Metagenomic site | No. of hits | Hit size (bp) | Identity (%) | E-value |
| HS5 | |||||||||
| 1 | 801 | hypothetical protein | AAMa | Station 365 (Antartic) | 1 | 233 | 96 | 10−100 | |
| 2 | 549 | hypothetical protein | - | - | - | - | - | - | |
| 3 | 3690 | FIG00912888: hypothetical protein | AAM | Station 354 (Antartic) | 3 | 364 | 96 | 10−157 | |
| Station 365 (Antartic) | 348 | 93 | 10−133 | ||||||
| Station 365 (Antartic) | 530 | ||||||||
| 4 | 3741 | putative type II restriction enzyme methylase subunit | AAM | Station 236 (Antartic) | 2 | 251 | 94 | 10−102 | |
| Station 365 (Antartic) | 530 | ||||||||
| GOSb | Fernandina Island (Galapagos) | 1 | 930 | 85 | 0.0 | ||||
| 5 | 2304 | hypothetical protein | GOS | Fernandina Island (Galapagos) | 1 | 996 | 83 | 0.0 | |
| 6 | 2043 | ATP-dependent protease La | - | - | - | - | - | - | |
| 7 | 771 | hypothetical protein | - | - | - | - | - | - | |
| 8 | 816 | hypothetical protein | - | - | - | - | - | - | |
| HS4 | |||||||||
| 1 | 1617 | ATPase involved in DNA repair | - | - | - | - | - | - | |
| 2 | 762 | Transposase ISTB | GOS | Sargasso Station 11 | 1 | 756 | 97 | 0.0 | |
| 3 | 1455 | Transposase IstA | GOS | Sargasso Station 11 | 1 | 1017 | 99 | 0.0 | |
| 4 | 1080 | ATPase involved in DNA repair | - | - | - | - | - | - |
a
AAM, Antatic Aquatic Metagenome.
b
GOS, Global Ocean Sampling Expedition.