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. 2014 Sep 29;9(9):e109857. doi: 10.1371/journal.pone.0109857

Figure 7. GSK-3 knockdown does not affect adult A. fluviatilis engorgement and development.

Figure 7

GSK-3 knockdown does not affect adult A. fluviatilis engorgement and development. Unfed Wol+ females were injected with 1 mg of either unrelated double-stranded RNA (b-gal, dashed bar) or double-stranded RNA specific for GSK-3 (black bar) and fed on blood 24 h after injection. RNA was extracted three days after the blood meal to confirm silencing. After an additional 3 days, the mosquito abdomen width was measured in both groups under a stereomicroscope. (A) The confirmation of silencing by real-time PCR. (B) Photos representing the graph are shown in C. The results are expressed as the mean 6 S.E. of three independent experiments, in triplicates. (Paired T’ test, p value, 0.05 was considered statistically significant).