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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Stem Cells. 2014 May;32(5):1149–1160. doi: 10.1002/stem.1609

Figure 3.

Figure 3

STAT3 S727 phosphorylation promotes neuronal differentiation of mouse embryonic stem cell (mESCs). (A): Immunofluorescence staining of different STAT3−/− + destabilizing domain (DD)-STAT3 transgenic mESCs with neural precursor cell marker Nestin (green) and neuron marker Tuj1 (red). Nuclei were stained with DAPI (blue). Cells were fixed and stained with above antibodies on day 18 of differentiation. (B): Real-time polymerase chain reaction analysis of induction of neural precursor genes (sox1 and nestin) in EB differentiation of STAT3−/− + DD-STAT3-WT and STAT3−/− + DD-STAT3-S727A cells. Oct4 is a marker for undifferentiated mESCs remaining in the population. (C): Immunofluorescence staining of STAT3−/− + DD-STAT3-S727D mESCs. (D): Quantitative comparison of the pixel intensity among STAT3−/− mESCs expressing various versions of DD-STAT3. The measurements were averaged on at least two different sets of images. (A, B): Scale bars 5 50 μm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; DD, destabilizing domain; KO, knock out; mESC, mouse embryonic stem cell; WT, wild type.