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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Mutat Res. 2014 Oct;768:35–46. doi: 10.1016/j.mrfmmm.2014.05.002

Figure 3. Silibinin inhibits fibronectin- and CAFs-induced invasiveness in PC3 cells.

Figure 3

(A) The bottom chambers of Transwell were filled with RPMI media containing 0.5% FBS supplemented with BSA or fibronectin (40 μg/ml), and in the top chambers PC3 cells (2 × 104) were seeded in RPMI media containing 0.5% FBS with or without silibinin (50 and 100 μM). After 10 h, PC3 cells invasiveness was measured. (B) CAF cells were grown in the bottom chambers of Transwell with or without silibinin (100 μM), and PC-3 cells (2 × 104) were seeded in the top chambers with or without silibinin (100 μM). After 10 hrs, PC3 cells invasiveness was measured. Cell invasion data shown are means±SEM of three samples. Representative photomicrographs are shown at 100×. Abbreviations: BSA: Bovine serum albumin; FN: Fibronectin; SB: Silibinin; CAF: Cancer-associated fibroblasts. *, p≤0.001; #, p≤0.01; $, p≤0.05