Skip to main content
. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Mutat Res. 2014 Oct;768:35–46. doi: 10.1016/j.mrfmmm.2014.05.002

Figure 4. Silibinin inhibits fibronectin-induced dissemination of PC3-luc cells in athymic nude mice.

Figure 4

(A-C) PC3 cells were cultured in RPMI media (with 0.5% FBS) for 24 hrs; thereafter cells were collected and re-plated on fibronectin (5 μg/ml) coated plates with or without silibinin (200 μM). PC3 cells plated on BSA (5 μg/ml) coated plates served as relevant control. After 24 hrs, cells were harvested, and 7 × 105 cells were injected in each male athymic nude mouse. Mice were imaged 6 hrs later as described in the ‘Materials and methods’. Representative mice images as well as bioluminescence intensity scale bar for each image are presented. Abbreviations: BSA: Bovine serum albumin; FN: Fibronectin; SB: Silibinin