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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: J Immunol. 2012 May 21;188(12):6407–6417. doi: 10.4049/jimmunol.1102492

FIGURE 3.

FIGURE 3

Chimeric protein generates elevated levels of high-avidity Abs in addition to NO. (A) Total IgG serum Ab responses against CS protein as measured by ELISA. (B) IgG isotypes induced by immunizations. Levels of IgG1 and IgG2a Abs generated by the vaccine regimes are shown. Control groups represent the animals receiving empty DNA or PBS for priming and MVA as boost. (C) Avidity indices of the Abs against CS protein. The avidity index was arbitrarily considered as the molarity of urea required to reduce the initial absorbance by 50% (i.e., log10 50% = 1.69). (D) Splenocytes from vaccinated groups harvested after 53 d after boost were incubated with 5 µg/ml recombinant CS protein for a period of 48 h. Amount of NO in control groups was deducted from DNA-CS- and protein-primed groups. Data are expressed as means ± SEM of triplicate observation (n = 4 mice/group) and are representative of two independent experiments. *p < 0.05, **p < 0.005, ***p < 0.0005.