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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: J Immunol. 2012 May 21;188(12):6407–6417. doi: 10.4049/jimmunol.1102492

FIGURE 4.

FIGURE 4

Enhanced immunogenicity of vaccine regimen based on chimeric protein. BALB/c mice were primed intradermally with DNA or proteins and boosted with i.p administration of 2 × 107 PFU MVA-CS after 2 wk. Splenocytes were stimulated with CS peptide SYVPSAEQI. The primary immune responses were analyzed on day 14 by (A) IFN-γ ELISPOT assay and (B) CD8+ cells secreting IFN-γ, TNF-α, and IL-2 by polychromatic flow cytometry. Memory responses were analyzed on day 53 after boost (C) IFN-γ ELISPOT assay and (D) memory CD8 analysis using CD44 and CD62L memory markers. Pie charts represent the polyfunctionality of CD8+ T cells secreting single, double, and triple cytokines. Data are expressed as means ± SEM of triplicate observations (n = 4 mice/group) and are representative of two independent experiments. *p < 0.05, **p < 0.005, ***p < 0.0005.