Table 1.
Primer sequences used in real-time PCR assays in colonic tissue
| Gene | Sequence 5′–3′ | Annealing T (°C) |
|---|---|---|
| GAPDH | FW: CCATCACCATCTTCCAGGAG | 60 |
| RV: CCTGCTTCACCACCTTCTTG | ||
| IL-1β | FW: GATCTTTGAAGAAGAGCCCG | 59 |
| RV: AACTATGTCCCGACCATTGC | ||
| IL-6 | FW: CTTCCAGCCAGTTGCCTTCTTG | 60 |
| RV: TGGTCTGTTGTGGGTGGTATCC | ||
| IL-12 | FW: ACGCTACCTCCTCTTCTTG | 60 |
| RV: ATGTCGTCCGTGGTCTTC | ||
| TNF-α | FW: GTCTTTGAGATCCATGCCATTG | 57 |
| RV: AGACCCTCACACTCAGATCA | ||
| CINC-1 | FW: CCGAAGTCATAGCCACACTCAAG | 60 |
| RV: TCACCAGACAGACGCCATCG | ||
| ICAM-1 | FW: GTGAACTGCTCTTCCTCTTG | 60 |
| RV: AGTGGTCTGCTGTCTTCC | ||
| MCP-1 | FW: TCTTCCTCCACCACTATGC | 60 |
| RV: TCTCCAGCCGACTCFATTG | ||
| Villin | FW: TGCTACCTGCTGCTCTATACCTAC | 59 |
| RV: CTGGCTCGTCGTTGTACTTCTG | ||
| TFF-3 | FW: ATGGAGACCAGAGCCTTCTG | 60 |
| RV: ACAGCCTTGTGCTGACTGTA | ||
| MUC-2 | FW: ACCACCATTACCACCACCTCAG | 60 |
| RV: CGATCACCACCATTGCCACTG | ||
| MUC-3 | FW: CACAAAGGCAAGAGTCCAGA | 60 |
| RV: ACTGTCCTTGGTGCTGCTGAATG |
Abbreviations: CINC, cytokine-induced neutrophil chemoattractant; FW, forward; GADPH, glyceraldehyde-3-phosphate dehydrogenase; ICAM, intercellular adhesion molecule; IL, interleukin; MCP, monocyte chemoattractant protein; MUC, mucin; PCR, polymerase chain reaction; RV, reverse; T, temperature; TFF, trefoil factor; TNF, tumor necrosis factor.