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Definition of viral titers. (a) pAdenoX encoding human bmp2 was identified using 2% agarose gel electrophoresis after PCR amplification with two primer sets. Lane 1: λ-HindIII digest; lane 2: φ X 174-HaeIII digest; lane 3: full sequence human bmp2 (1191 bp); lane 4: partial sequence human bmp2 (225 bp). (b) HEK293 cells were infected with adenovirus encoding bmp2, which demonstrated a titer of 5.85 × 106 ifu/mL.
