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. 2014 Nov;146:46–54. doi: 10.1016/j.ultramic.2014.05.009

Fig. 2.

Fig. 2

Modification of Cryostage2 (MPI Biochemistry, Martinsried) to enable correlative fluorescence cryo-measurements of vitreous samples, on dedicated grids (IFR-1 or HZB-2) for the soft X-ray cryo-microscope at the beamline U41-FSGM of BESSY II (Berlin). (A) Overview of the Cryostage2 mounted in a motorized microscope stage of an inverted light microscope. (B) Modified specimen slider of the Cryostage2 (left, with HZB-2 gold grids in position 2 and 4) in comparison to the original slider for 3.05 mm standard electron microscopic grids only (right). (C) Loading and (D) imaging position of a HZB-2 gold grid in the sample chamber of the Cryostage2.