Table 4.

Selection pressure analysis of NS1 protein and NEP of H1N1 pdm (09) virus using SLAC, FEL, REL, MEME, and FUBAR methods at (http://www.datamonkey.org).

Protein	Codon∗	SLAC dN-dS	SLAC P value	FEL dN-dS	FEL P value	REL dN-dS	REL Bayes factor	MEME ω+	MEME P value	FUBAR dN-dS	FUBAR post. pr.
NS1	55	17.614	0.131	73.794	0.056	3.898	3779.510	>100	0.069	3.151	0.989
	108	5.330	0.459	19.133	0.208	3.149	11.971	>100	0.251	0.286	0.769
	123	5.399	0.447	24.424	0.142	3.558	22.722	>100	0.219	0.542	0.813
	129	13.333	0.142	62.522	0.043	3.978	19238.300	>100	0.074	2.993	0.992
	145	8.103	0.630	37.071	0.318	3.776	514.262	>100	0.328	1.285	0.924
	147	7.491	0.232	34.083	0.084	3.828	45.133	>100	0.101	1.081	0.880
	205	8.870	0.438	33.193	0.162	3.928	2940.990	>100	0.171	1.042	0.922
NEP	49	6.729	0.457	134.502	0.158	0.260	2.013	>100	0.183	1.846	0.926

Significance value (SLAC P value = 0.5, FEL P value = 0.25, REL Bayes factor = 50, MEME P value = 0.1, FUBAR posterior probability = 0.9).

∗The sites found under positive selection by at least two methods are shown.

Sites present in NS1 host factor interaction domains are highlighted with bold font.