Table 5.

Cluster specific amino acid changes were seen in all the sequences of cluster and were absent from sequences of all other clusters.

Clusters	Sites	Cluster specific amino acid changes
KOL 507 (2007, 2009)	13	D26, N53, C59, L85, V95, N143 (99%), T145, N171, T209, F214, T216, T217, T226
KOL 596 (2007, 2009)	7	P3, R59, A60, S103, I106, A171, I226
KOL 989 (2007–2009)	19	V23, R41, A56, H59, K67, V82, T84, I95, L98, N101, E112, M129 (80%), I144, V145, I171, K196, R224, A226, K229
NIV 6196 (2009–2013)	28	M6, F22, N25 (99%), G26, L59, W67, S74, T76, R78, I81, T86, S91, R108 (99%), I111 (90%), I112, L119, V123 (85%), V129, N139, Y171, I198, N205 (77%), C206, D207, S213, P215, E217, ∗220

Some cluster specific changes were seen in variable percentage of sequences in cluster and those absent from all other clusters are highlighted with italic font.