Table 4.

Summary of non­mammalian study characteristics.

Source [source ID]	Species	Time point(s) of outcome measurement	Outcome measure	Route of exposure	Period of exposure	PFOA dose range	No. of doses administereda	No. of offspring
Hagenaars et al. 2011 [59]	Zebrafish	120 hpf (posthatching)	Length	Egg immersion	Spawning, 120 hpf	15–250 mg/L	8	292
Wang et al. 2010 [86]	Fruit fly	30, 48, 72, 96, and 110 ael (larval stages)	Lengthb	Food	Egg laying, 110 ael	100–500 μM	2	378
Wang et al. 2010 [86]	Fruit fly	Pupae	Weight	Food	Egg laying, white pupae stage	100–500 μM	2	98
Pinkas et al. 2010 [187]	Chicken	Hatchling	Weight	Egg injection	Single treatment at incubation day 0	5–10 mg/kg egg	2	52
O’Brien et al. 2009 [236]	Chicken	Embryo at pipping star or day 22, whichever came first	Weight	Egg injection	Single treatment at incubation day 0	0.01–10 mg/kg egg	4c	37
Jiang et al. 2012 [3926]	Chicken	Embryonic day 19	Yolk-free body weight	Egg injection	Single treatment at incubation day 0	0.5–2 mg/kg egg	2c	40
Jiang et al. 2012 [3926]	Chicken	16–24 hr posthatching	Yolk-free body weight and crown to rump length	Egg injection	Single treatment at incubation day 0	0.5–2 mg/kg egg	2c	68
Spachmo and Arukwe 2012 [3932]	Salmon	Study days 21, 35, 49, 56 (larval stages posthatching)	Length and dry weight	Egg immersion	Egg stage, day 48	100 μg/L	1	80
Abbreviations: ael, hours after egg laying; hpf, hours postfertilization. aExcludes control groups; study used one control group unless otherwise specified. bLength measurements provided by study author (used to calculate volume outcome reported in study). cIncluded two control groups.