Figure 4. Effects of Cl, Met and MetCl therapy on cardiomyocyte contractility (A–C) and Ca²⁺ handling (D–F) during mechanical unloading (MUHF), measured using Indo-1 and Ionoptix system.

Cl's enhancement of MU-induced recovery of speed of sarcomeric contraction (B) and Met's antagonism of MU-induced improvement in speed of relaxation (C) are shown. Cl and Met's enhancement of MU-induced recovery of Ca²⁺ transient amplitude (D), speed of Ca²⁺ release (E) and SR Ca²⁺ content (F), and lack of enhancement during combined MetCl therapy is shown. *P<0.05, **P<0.01 and ***P<0.001. Representative traces of sarcomeric contractions (G) and Ca²⁺ transients (H) are shown.