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. 2014 Sep 30;9(9):e106842. doi: 10.1371/journal.pone.0106842

Figure 6. Dose-response effects of TGF-beta on TMEM16A, CFTR, e-cadherin, and vimentin in T84 cells.

Figure 6

T84 cells were treated with TGF-beta (vehicle control, 0.1, 1.0, and10 ng/ml) for 48 h, followed by Ussing chamber and immunoblot as described in Methods. (A and B) CaCC (*P<0.003, **P<0.01) and CFTR (*P<0.0001) currents were reduced by TGF-beta treatment at every dose used. (C and D) TMEM16A (*P<0.01, **P<0.005) and CFTR (*P<0.05, **P<0.0001) expression was suppressed by TGF-beta treatment at every dose used. E-cadherin expression (E) was decreased (*P<0.011) and vimentin expression (F) was increased (*P<0.05) with 1 ng/ml or greater TGF-beta treatment.