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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: FEBS J. 2014 Aug 14;281(19):4394–4410. doi: 10.1111/febs.12947

Fig. 3.

Fig. 3

Pre-steady-state burst kinetics of hpol κ and mutants under enzyme-limiting conditions. Each reaction was performed by rapid mixing of a pre-formed 100 nM 24-/36-mer DNA duplex (X: G or 8-oxoG)-hpol κ mixture with 1.0 mM dNTP in the presence of 50 mM Tris-HCl buffer (pH 7.5) containing 3 mM DTT, 50 μg BSA mL−1, and 5% (v/v) glycerol at 23 °C. Reactions were quenched with 0.5 M EDTA. (A) WT hpol κ (25 nM); (B) W214Y/W392H (25 nM); (C) Y50W (15 nM); (D) T408W (14 nM). The different base pairs are: G:dCTP (●), 8-oxoG:dATP (■), and 8-oxoG:dCTP (◆). Results were fit to the burst equation (Eq. 1).