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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Cell Physiol. 2015 Jan;230(1):63–70. doi: 10.1002/jcp.24681

Fig. 1. hESC pluripotency is maintained in mTeSR1 culture medium and treating with retinoic acid triggers differentiation.

Fig. 1

A: Western blot showing the presence of pluripotency markers, Oct4 and Nanog, in H9 hESC cultures grown for 1, 3, and 5 days in medium designed to maintain pluripotency, mTeSR1. The right side of the figure shows that Oct4 and Nanog protein expression is lost at 3 and 5 days in culture medium that contains retinoic acid, indicating that cells have begun to differentiate. B: RT-PCR for differentiation markers Gata4 and Pax6 shows that these genes are much more highly expressed in cells grown in RA-containing medium compared to those cultured to retain pluripotency.