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. Author manuscript; available in PMC: 2015 Sep 29.
Published in final edited form as: Dev Cell. 2014 Sep 11;30(6):660–672. doi: 10.1016/j.devcel.2014.07.008

Figure 2. Cpeb4 is required for terminal erythroid differentiation.

Figure 2

(A) Cpeb4 mRNA and Cpeb4 and Gapdh protein levels in shRNA knockdown cells and the shRNA control cells. The arrow indicates Cpeb4 protein, and # indicates a non-specific band. (B) Growth curves of Cpeb4 knockdown cells and the shRNA control cells in the differentiation medium. (C) Measurement of the number of cell divisions at 24 hours and 48 hours in the differentiation medium. (D–E) The cell cycle profiles of Cpeb4 knockdown cells and the shRNA control cells. (F–G) Apoptotic status of Cpeb4 knockdown cells and the shRNA control cells was measured by annexin-V staining. (H–I) Quantification of genes induced and repressed in terminal erythroid differentiation by real-time RT-PCR. All the results are from 3 independent measurements and presented as mean ± SD. * indicates p < 0.01 compared to the corresponding control (two-tailed Student’s t test). See also Figure S3.