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. Author manuscript; available in PMC: 2015 Sep 29.
Published in final edited form as: Dev Cell. 2014 Sep 11;30(6):660–672. doi: 10.1016/j.devcel.2014.07.008

Figure 3. Cpeb4 knockdown blocks the generation of enucleated reticulocytes.

Figure 3

(A) The generation of enucleated reticulocytes was analyzed in the Cpeb4 knockdown cells and the shRNA control cells. Staining was with Ter119 and the Hoechst DNA- binding dye. (B) The expression of cell surface markers Ter119 and CD71 was analyzed in Cpeb4 knockdown cells and the shRNA control cells. (C–D) Quantifications of relative efficiencies of enucleation and the percentages of Ter119 and CD71 double positive cells in the Cpeb4 knockdown cells and the shRNA control cells. All the results are from 3 independent measurements and presented as mean ± SD. * indicates p < 0.01 using two-tailed Student’s t test.