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. Author manuscript; available in PMC: 2015 Sep 29.
Published in final edited form as: Dev Cell. 2014 Sep 11;30(6):660–672. doi: 10.1016/j.devcel.2014.07.008

Figure 7. Cpeb4 is functionally important in vivo.

Figure 7

(A) Structures of Cpeb4 genomic region in wild type and Cpeb4 knockout mouse. P1, P2, and P3 are PCR primers for genotyping. (B) Genotyping of wildtype (+/+), Cpeb4 heterozygous (+/−), and Cpeb4 knockout (−/−) mice by PCR. (C) Western blot of E14.5 total fetal liver cells isolated from wildtype, Cpeb4 heterozygous, and Cpeb4 homozygous knockout embryos. (D) Genotyping of E14.5-E15.5 embryos from a mating of Cpeb4 heterozygotes. (E) Genotyping of 2–3 week old mice from matings of Cpeb4 heterozygotes. (F) The quantification of Ter119+ cells in fetal livers from E14.5 embryos. The y axle is the scale relative to a known amount of added beads. * indicates p < 0.05 using two-tailed Student’s t test. (G) CD71 and Ter119 staining of total E14.5 fetal liver cells. (H) CD71 expression levels in Ter119+ cells from E14.5 mouse fetal liver. The black arrow indicates high CD71 level; the red arrow indicates low CD71 level. The results of (G, H) are representative of E14.5 embryos from 4 sets of matings of Cpeb4 heterozygotes.