Skip to main content
. Author manuscript; available in PMC: 2015 Sep 15.
Published in final edited form as: Gen Comp Endocrinol. 2014 Aug 1;206:30–42. doi: 10.1016/j.ygcen.2014.07.022

Fig. 5. Constitutive transcriptional activity of HR97 receptors.<.

Fig. 5

br>A) HepG2 cells were transfected with 0.02 mg of pBIND-GAL4-97a/b/g-DEF chimeric plasmid and 0.1 μg of the reporter plasmid, pG5luc. Control cells were transfected with pG5luc only and transactivation activity was measured as described in the Materials and Methods. Only HR97b increases constitutive activity. (B–D) Cells were transfected with 0, 0.01, 0.02, 0.04 or 0.08 μg pBIND-GAL4-97a/b/g-DEF chimeric plasmid supplemented with empty pBIND-GAL4 vector to ensure all wells contained 0.08μg of total expression plasmid and luciferase activity measured. Assays were performed two times. An asterisk (p < 0.05) or two asterisks (p < 0.01) indicates statistical significance as determine by ANOVA followed by Dunnett’s multiple comparison test with GraphPad Prizm 4.0 (n = 3).