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. Author manuscript; available in PMC: 2015 Sep 12.
Published in final edited form as: Oncogene. 2014 Mar 31;34(11):1341–1353. doi: 10.1038/onc.2014.72

Figure 2.

Figure 2

Piperlongumine inhibits ligand-induced Stat3 phosphorylation in Kasumi1 cells. Serum-starved (1 hour) Kasumi-1 cells, pre-incubated with PL/DMSO (1 hour), were treated with G-CSF (10 ng/ml, 15′). Cells were permeabilized and stained with Alexa647-pStat3, and analyzed on BD LSR2. FCS files were uploaded to Cytobank for pStat3 quantitation. Histograms depicting pStat3 levels (A) are shown. These mean fluorescence (pStat3) levels were plotted as function of the Log [M] PL, and IC50 calculated using GraphPad. 2B shows IC50 curve from representative experiment.