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. Author manuscript; available in PMC: 2015 Sep 12.

Published in final edited form as: Oncogene. 2014 Mar 31;34(11):1341–1353. doi: 10.1038/onc.2014.72

Piperlongumine blocks growth of breast cancer cell lines with activated Stat3 and induces apoptosis. A. MDA-MB-231 cells (left column) and MDA-MB-468 cells (right column) were treated in ultra-low attachment plates with complete media ± piperlongumine (0/0.1/0.3/1/3/10/100 μM, 72hrs) and photographed. Shown are representative wells for each treatment. B. Cells were treated as above and viable cells were quantitated using MTT. Relative % viability was measured by (viability after any treatment ÷ viability of untreated cells × 100) and plotted as a function of Log [M] PL, and IC50 values calculated using GraphPad. Data show representative experiments from ≥2 replicates. C. Piperlongumine blocks anchorage dependent growth of MDA-MB-231, MDA-MB-468 and Hs 578T cells. Cells were cultured for 24–48 hrs in complete DMEM with 10% FBS ± piperlongumine (0/0.1/0.3/1/3/10/100 μM) in cell-culture-treated 96-well plates. Viable cells quantitated using MTT. Relative % viability and IC50s were calculated as above. Data show representative experiments from ≥ 2 replicates. D. Proteins from MDA-MB-468 treated with 30μM PL (15′ - 24 hrs) were examined for cleaved caspase 3 and GAPDH by Luminex. GAPDH-normalized active caspase 3 values expressed as a percentage of the pre-treatment value (±SD) plotted along Y-axis.

Piperlongumine blocks growth of breast cancer cell lines with activated Stat3 and induces apoptosis. A. MDA-MB-231 cells (left column) and MDA-MB-468 cells (right column) were treated in ultra-low attachment plates with complete media ± piperlongumine (0/0.1/0.3/1/3/10/100 μM, 72hrs) and photographed. Shown are representative wells for each treatment. B. Cells were treated as above and viable cells were quantitated using MTT. Relative % viability was measured by (viability after any treatment ÷ viability of untreated cells × 100) and plotted as a function of Log [M] PL, and IC50 values calculated using GraphPad. Data show representative experiments from ≥2 replicates. C. Piperlongumine blocks anchorage dependent growth of MDA-MB-231, MDA-MB-468 and Hs 578T cells. Cells were cultured for 24–48 hrs in complete DMEM with 10% FBS ± piperlongumine (0/0.1/0.3/1/3/10/100 μM) in cell-culture-treated 96-well plates. Viable cells quantitated using MTT. Relative % viability and IC50s were calculated as above. Data show representative experiments from ≥ 2 replicates. D. Proteins from MDA-MB-468 treated with 30μM PL (15′ - 24 hrs) were examined for cleaved caspase 3 and GAPDH by Luminex. GAPDH-normalized active caspase 3 values expressed as a percentage of the pre-treatment value (±SD) plotted along Y-axis.