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. Author manuscript; available in PMC: 2015 Nov 28.
Published in final edited form as: Cancer Lett. 2014 Aug 27;354(2):299–310. doi: 10.1016/j.canlet.2014.08.032

Fig. 5.

Fig. 5

DJ4 inhibits migration and invasion of cancer cells. (A and B) Percent migration relative to control treated cells was determined by performing scratch assays in (A) lung adenocarcinoma (H522), breast (MDA-MB-231), pancreatic (PANC-1) and (B) melanoma (A375M) cancer cell lines. Cells were treated with DJ4 or DMSO for 24 h then monolayers were wounded and migration was allowed to proceed for 6–7 h (MDA-MB-231 and PANC-1) and 11–12 h (A375M) in the presence of DJ4 or DMSO. Error bars indicate SEM (n = 3, except for MDA-MB-231 where n = 2). ***P < 0.0001. (C and D) A549 cells treated with DJ4, Y-27632 or DMSO for 24 h. Monolayers were then wounded and migration was allowed to proceed for 9 h in treatment-free medium (C). Percent migration relative to control treated cells was determined and quantitative analysis of migration of A549 cells is presented in (D). (E and F) MDA-MB-231 cells were treated with DMSO or the indicated concentrations of DJ4 and allowed to invade through Matrigel coated membranes (8.0 µm) for 48 h. The error bars indicate SEM from two independent experiments.