(A) Typical gene organization of vertebrate mtDNA. (B) Gene organization of Tetrabrachium ocellatum mtDNA, in which the corrections to the original genbank file are highlighted. Light-strand encoded genes are underlined. Gene sizes are drawn relative to genome length. Gene abbreviations used are: ND1–6, NADH dehydrogenase subunits 1–6; COX1–3, cytochrome oxidase subunits I–III; ATP6 and ATP8, ATPase subunits 6 and 8; CYTB, cytochrome b; and one-letter codes of amino acids, tRNA genes specifying them (L1 and L2 for leucine tRNA genes specifying, respectively, UUR and CUN codons and S1 and S2 for serine tRNA genes specifying, respectively, AGY and UCN codons). CR, OriH, and OriL stand for the control region, the heavy-strand replication origin, and the light-strand replication origin, respectively. The bars connecting both mtDNA linear representations indicate relevant gene rearrangements (light grey) and inversions (dark grey).