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. 2014 Sep 23;3:550. doi: 10.1186/2193-1801-3-550

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© Nakamura et al.; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Effect of MβCD on the distribution of flotillin-1 and cholesterol in microdomain fractions. After 30 min incubation of fibroblast with serum-free DMEM in the presence of 10 mM MβCD (MβCD (+)) or in the absence of MβCD (MβCD (-)), cell lysates were subjected to ultracentrifugation (268,000 × g for 4 h at 4°C) and split into nine fractions. (A) Dot blot analyses of flotillin-1. (B) Semi-quantitative TLC analyses of cholesterol. Open circles, no treatment; closed circles, treatment with MβCD. Values are the means ± S.D. (n = 3).