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. 2014 Sep 30;9(9):e107637. doi: 10.1371/journal.pone.0107637

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© 2014 An et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Semi-quantitative RT-PCR analysis of ABS5/T5L1 gene expression in various tissues: roots, two-week-old seedlings, rosette leaves, stems, cauline leaves, siliques and flowers. The expression of Actin2 was used as a control. B. Nuclear localization of ABS5-GFP fusion protein in Arabidopsis leaf protoplasts. Wild type leaf protoplasts were transformed with P_35S::GFP or P_35S::ABS5-GFP. A single representative protoplast was shown for each transformation. Nuclei were specifically stained by the fluorescent dye Hoechst33342. Hoechst33342, GFP, chlorophyll autofluorescence signals were monitored by fluorescence microscopy. Bright field (BF) images served as controls for protoplast integrity. Bars: 10 µm.