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. Author manuscript; available in PMC: 2015 Sep 29.
Published in final edited form as: Dev Cell. 2014 Sep 18;30(6):731–745. doi: 10.1016/j.devcel.2014.08.007

Fig7. Centrosomes and polarity cues both help maintain spindle orientation.

Fig7

(A,G) Minimal apoptosis in control ap>GFP discs. (B,G) Single RNAi against sas-4 or the spindle orientation pathway component mud (C) elevate apoptosis, but have no appreciable effects on adult wings. (D,G) Co-expressing sas-4 and mud RNAi significantly increased apoptosis and severely disrupted adult wings. (E) RNAi against pins does not increase apoptosis. (F) Apoptosis increased after Sas-4/Pins double knockdown. This also perturbs adult wing development. (H) Model of en face view of wing disc. Spindle orientation analyzed in the pouch (green). Dotted line=Example of location of reconstructed cross-sectional views in I,K. Right=Diagrammatic examples (anaphase nuclei, red; MT in black; Apicobasal polarity indicated). Pink boxes=close-ups in I,K. (I,J) In WT discs, anaphase nuclei (PH3, red) divide in parallel with the epithelial sheet (n=43). (K,L) In sas-4 mutants, we found an increased mean and expanded distribution of division angles (blue arrow; n=34). See also Fig S3,S7. Scale bars A-F=50 μm; I,K=10μm.