Fig. 5.

Inhibition of lanosterol binding to C. albicans CYP51 by azole fungicides. Type I difference spectra were measured during the progressive titration of 10 μM CYP51 with lanosterol in the absence and presence of azole fungicides. Lineweaver-Burk plots were constructed from the type I binding spectra obtained in order to compare lanosterol binding in the absence (black circle) and presence (white circle) of 4 μM voriconazole, 4 μM prothioconazole-desthio and 100 μM prothioconazole. One representative example of each experiment is shown, although all experiments were performed in triplicate