Fig. 6.

Azole IC₅₀ determinations. a The CYP51 activities of 2.5 μM wild-type (black circle), S279F (white circle) and I471T (◉) C. albicans CYP51 were determined at fluconazole concentrations ranging from 0 to 4 μM. A relative velocity of 1.0 corresponds to the velocity observed with the wild-type enzyme in the absence of fluconazole (0.062 nmol min⁻¹). b The CYP51 activities of 2.5 μM wild-type C. albicans CYP51 were determined at voriconazole (black circle) and prothioconazole-desthio (◉) concentrations ranged from 0 to 4 μM and prothioconazole (white circle) concentrations ranged from 0 to 100 μM. Relative velocities of 1.0 equate to actual velocities of 0.080, 0.098 and 0.087 nmol min⁻¹ for the IC₅₀ determinations with voriconazole, prothioconazole and prothioconazole-desthio, respectively. Mean values from three replicates are shown along with associated standard error bars