Figure 4.

Early [1 week post-intracranial injection (wp-ic.)] therapeutic blockade against CTLA-4, PD-L1 and IDO decreases Treg levels and increases IFN-γ⁺ Tc in brain tumors. Wild-type (WT) mice ic. injected with 4×10⁵ GL261 cells and left untreated or administered D1-MT, CTLA-4 mAb (clone 9H10) and/or PD-L1 mAbs (clone 10F.9G2). The frequency of (A) CD4⁺FoxP3⁺ and CD4⁺FoxP3⁺CD44⁺ Treg, (B) CD8⁺ and CD8⁺CD44⁺ Tc or (C) CD4⁺IFN-γ⁺ and CD8⁺IFN-γ⁺ effector-Tonv and -Tc, respectively, isolated from the brain, bilateral deep and superficial cervical draining lymph nodes (cLN) and the spleen of glioma-bearing mice at 3 wpic. left untreated, or administered D1-MT, CTLA-4 and/or PD-L1 (n = 5/group). All T cell populations were initially identified by the expression of CD3. *P < 0.05; **P < 0.01.