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. 2014 Sep 30;9(9):e107981. doi: 10.1371/journal.pone.0107981

Figure 2. The impact of the mutagenesis of the lysine residues on the total PT modifications.

Figure 2

A. The plasmids pWHU668-pWHU674 possessing mutated lysine residues were expressed in E. coli DH10B and XTG102, and the DNA PT frequencies were measured by LC-MS/MS. The columns and error bars represent the mean ± SD; n = 3, biological triplicates. B. qRT-PCR expression analysis of the dndE genes in E. coli DH10B (white bars) and XTG102 (grey bars). The expression levels were normalized to the transcript levels of GAPDH. The columns and error bars represent the mean ± SD; n = 3, biological triplicates. C. PT modifications were normalized to the average dndE expression levels.