Table 4. PT modifications in XTG102 harboring pJTU1238 and its derivatives.
Mutations | Strains | PT modifications per 106 nt | ||
d(GPSA) | d(GPST) | Total PT | ||
wild type | XTG102(pJTU1238) | 374±5 | 299±5 | 673±10 |
K17A | XTG102 (pWHU668) | 383±3 | 264±5 | 646±8 |
K18A | XTG102 (pWHU669) | 383±8 | 251±4 | 635±11 |
K20A | XTG102 (pWHU670) | 392±4 | 265±12 | 657±10 |
K53A | XTG102 (pWHU672) | 398±8 | 377±16 | 775±9 |
K87A | XTG102 (pWHU673) | 451±11 | 417±14 | 868±24 |
K91A | XTG102 (pWHU674) | 410±8 | 390±8 | 800±3 |
K18A+K20A | XTG102 (pWHU671) | 297±5 | 233±9 | 530±14 |
ΔdndE | XTG102 (pWHU676) | ND | ND | ND |
Values represent the mean ± SD for biological triplicates; ND, not detected.