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. 2014 Sep 30;9(9):e108373. doi: 10.1371/journal.pone.0108373

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© 2014 Nagano et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Total leukocyte count of the thoracic lymph nodes. All visible thoracic lymph nodes were collected from the sensitized mice with the indicated PLCε genotype 1 day after the last challenge with vehicle alone or OVA (6 to 15 mice of each group), and subjected to isolation of leukocytes. Leukocyte number was determined using a hematocytometer. Data are expressed as the mean ± SD. ***, p<0.001. (B) Flowcytometric analysis of leukocytes. Collected leukocytes in (A) were further analyzed flowcytometrically for the expression of the indicated cell surface antigens. Data are expressed as the mean ± SD. *, p<0.05; **, p<0.01. n.s., statistically not significant.