Table 1. Sampling and bacterial isolate designations.
Site Name and Location | Date ofCollection | WaterDepth (m) | WaterTemp (°C) | Colony No. | Host Coral | SampleType(s)1 | Bacterial IsolateDesignations2 , 3 , 4 | No. of IsolatesExamined |
Ft. Lauderdale, Broward County, FL, USA; 26° 11.35′ N, 80° 5.49′ W | 17-May-08 | 3.7 | 28 | FTL 1 | Montastraea cavernosa | BBD | BBD-FTL-1x | 109 (41 BBD, 23 BSML, 45 HSML) |
27-Jun-08 | 4.6 | 28 | FTL 2 | M. cavernosa | BBD | BBD-FTL-2x | ||
27-Jun-08 | 4.3 | 28 | FTL 3 | M. cavernosa | BBD | BBD-FTL-3x | ||
27-Jun-08 | 4.0 | 28 | FTL 4 | M. cavernosa | BBD | BBD-FTL-4x | ||
27-Jun-08 | 3.3 | 28 | FTL 5 | M. cavernosa | BBD | BBD-FTL-5x | ||
1-Aug-09 | 4.6 | 29 | FTL 6 | M. cavernosa | BBD, BSML | BBD-FTL-6x, BSML-FTL-6x | ||
31-Jul-10 | 3.7 | 30 | FTL 7 | M. cavernosa | BBD, BSML | BBD-FTL-7x, BSML-FTL-7x | ||
31-Jul-10 | 4.3 | 30 | FTL 8 | M. cavernosa | BBD, BSML | BBD-FTL-8x, BSML-FTL-8x | ||
31-Jul-10 | 4.6 | 30 | FTL 9 | Diploria strigosa | HSML | HSML- FTL-9x | ||
31-Jul-10 | 4.3 | 30 | FTL 10 | M. cavernosa | HSML | HSML- FTL-10x | ||
31-Jul-10 | 4.3 | 30 | FTL 11 | M. cavernosa | HSML | HSML- FTL-11x | ||
Algae Reef, Florida Keys, FL, USA; 25° 08.799′ N, 80° 17.579′ W | 24-Oct-09 | 6.7 | 25 | FLK 1 | D. strigosa | BBD, BSML | BBD-FLK-1x, BSML-FLK-1x5 | 27 (13 BBD, 14 BSML) |
Horseshoe Reef, Florida Keys, FL, USA; 25° 08.362′ N, 80° 17.641′W | 7-Sept-12, 30-Sept-12 | 3.7 | 29 | FLK 26 | Colpophyllia natans | BBD | BBD-FLK-1Mx, BBD-FLK-1Sx,BBD-FLK-2Mx, BBD-FLK-2Sx | 7 |
Water Factory 3, Curacao; 12° 06.779′ N, 68° 57.662′ W | 23-Feb-13 | 2.5 | 27 | CUR 1 | D. strigosa | BBD | BBD-CUR-3Mx, BBD-CUR-3Sx | 39 |
Horseshoe Reef, Lee Stocking Island, Bahamas; 23° 46.30′ N, 76° 5.33′ W | 9-Jul-04 | 8.2 | 29 | 216 | Siderastrea siderea | BBD | BBD-216-x | 5 |
7.3 | 28 | 217 | S. siderea | BBD | BBD-217-x | 4 |
BBD samples were obtained from a BBD mat sample; BSML samples were obtained from the SML of an apparently healthy area of a BBD-infected colony; HSML samples were obtained from the SML of an apparently healthy colony.
The “x” in the isolate designation refers to an individual isolate from the source colony (e.g., BBD-FTL-1a, BBD-FTL-1b, etc.).
The “M” in the isolate designation refers to isolates that were selected from a marine agar plate.
The “S” in the isolate designation refers to isolates that were selected from a sea water tryptone agar plate.
Includes BBD-216-1b, BBD-216-2d, BBD-216-3d, BBD-216-4a, BBD-216-4e, BBD-217-2b, BBD-217-2d, BBD-217-2g, and BBD-217-3m. Sampling and identification information available from [42], [43].
Two BBD samples were collected from the single colony of C. natans on separate days. Sample 1 and Sample 2 were collected on 27-Sept-12 and 30-Sept-12, respectively.