Table 3. Quorum sensing assays conducted in this study.
Chromobacterium violaceum CV026 Assay 1 | Agrobacterium tumefaciens NTL4(pZLR4) Assay1 | Vibrio harveyi BB170 Assay1 | ||||||
Site Name and Location | No. of IsolatesExamined | PatchTest | CFTest | BBD MatTest | PatchTest | CFTest | BBD MatTest | CulturedIsolates |
Ft. Lauderdale | 109 | + | + | + | + | + | + | + |
Algae Reef | 27 | + | + | + | + | + | + | + |
Horseshoe Reef, Florida Keys | 7 | + | NT | NT | NT | NT | NT | NT |
Water Factory 3, Curacao | 39 | + | NT | NT | NT | NT | NT | NT |
Horseshoe Reef, Lee Stocking Island | 9 | + | + | + | + | + | + | + |
BBD cyanobacteria | 8 | + | + | + | + | + | + | + |
“+” indicates that the assay was conducted; NT indicates that the assay was not conducted. Patch Test and CF Test refer to isolates and BBD Mat Test refers to intact, freshly collected BBD mat, all tested using the CV026 and NTL4 reporter strains. The BB170 assay utilized actively growing cultures of isolates only (see methods section).