Table 4. Reporter assay results and sequencing data for the eleven bacterial isolates that tested positive for AHL production.
| Chromobacterium violaceumCV026 Assay1 | Agrobacterium tumefaciensNTL4(pZLR4) Assay1 | ||||||
| BBD andCoral Isolates | Patch Test | CF Test | Patch Test | CF Assay | GenBankAccession No. | SequenceLength (bp) | Closest Relative in GenBank (% Similarity),Accession No. |
| BBD-FTL-6j | + | − | − | + | KF148598 | 1393 | 2 Vibrio rotiferianus strain : LMG 21460 (99%), NR042081 |
| BBD-FTL-8c | + | − | − | + | KF148599 | 1415 | 2 Vibrio rotiferianus strain : LMG 21460 (99%), NR042081 |
| BBD-FLK-1d | − | − | + | + | KF148596 | 1326 | Nautella italica strain : LMG 24365 (99%), NR042673 |
| BBD-CUR-3M8 | + | NT3 | NT4 | NT4 | KF 494427 | 1391 | Vibrio harveyi strain : NB0903 (99%), HM008704 |
| BBD-CUR-3S11 | + | NT3 | NT4 | NT4 | KF 494426 | 1396 | Vibrio rotiferianus strain : DB1 (99%), KC756840 |
| BSML-FTL-6w | − | − | + | − | KF148595 | 1326 | Ruegeria scottomollicae : LMG 24367 (96%), NR042675 |
| BSML-FTL-7l | ND5 | − | + | − | KF148600 | 1385 | Pseudoalteromonas phenolica strain O-BC30 (98%), NR028809 |
| HSML-FTL-9c | − | − | + | + | KF148601 | 1414 | Vibrio harveyi strain NCIMB 1280 (99%), NR043165 |
| HSML-FTL-9e | − | − | + | + | KF148602 | 1398 | Aliagarivorans marinus strain AAM1 (95%), NR044585 |
| HSML-FTL-9i | − | − | + | + | KF148603 | 1345 | Pseudoruegeria aquimaris strain SW-255 (96%), NR043932 |
| HSML-FTL-10a | ND5 | − | + | + | KF148597 | 1387 | Pseudoalteromonas luteoviolacea strain NCIMB 1893 (99%), NR026221 |
1
“+” indicates a positive assay result and a “−” indicates a negative result.
2
Although BBD-FTL-6j and BBD-FTL-8c shared the same closest relative in GenBank, these isolates showed differing colony morphologies and were isolated from two different BBD samples on three separate coral colonies.
3
Culture fluids of isolates not tested with the C. violaceum CV026 reporter strain.
4
Isolates not tested with the A. tumefaciens NTL4(pZLR4) reporter strain.
5
Not detectable. These isolates inhibited growth of the C. violaceum CV026 reporter strain in the patch tests, eliciting an area of clear, cell-free agar in the assay plate surrounding the bacterial patch. Additionally, isolate HSML-FTL-10a produced a dark purple pigment.