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. 2014 Sep 30;9(9):e108541. doi: 10.1371/journal.pone.0108541

Table 6. AHLs detected via LC-MS/MS after 24 hour incubation.

Isolate AHLs Detected in Liquid Culture1 , 3 , 4 AHLs Detected on Agar2 , 3 , 4 DPD Conc (µM)1 , 5
BBD-FTL-6j C6 (78%), 3OC4 (13%), 3 others (9%) C6 (74%), 3OC4 (11%) 3 others (15%) 0.99±0.32
BBD-FTL-8c C6 (70%), 3OC4 (11%), 6 others (9%) C6 (50%), 3OC6 (21%), C4 (13%), 4 others (16%) 0.94±0.09
BBD-FLK-1d C6 (69%), 3OHC10 (9%), 9 others (22%) C6 (61%), 7 others (39%) 1.08±0.28
BBD-CUR-3M8 C6 (92%), 3 others (8%) C6 (69%), 3OC4 (31%) 1.10±0.49
BBD-CUR-3S11 C6 (86%), 3 others (14%) C6 (80%), 3 others (20%) 1.38±0.19
BSML-FTL-6w C14 (45%), C10 (26%), 3OHC4 (23%) 3OC20 (5%) C10 (39%), 3OHC8 (23%), 3OHC4 (18%), 3 others (20%) 0.32±0.13
BSML-FTL-7l 26 AHLs measured (ranging from 0.6–14%) ND6 0.48±0.09
HSML-FTL-9c 3OC4 (52%), C14(31%), 3OC18∶1(12%), 3OC20 (5%) 3OHC9∶1 (79%), 3OHC4 (21%) 1.41±0.16
HSML-FTL-9e 3OHC10 (75%), 7 others (15%) C6 (65%), 3OHC10 (22%), 4 others (13%) 0.37±0.02
HSML-FTL-9i 3OHC10 (70%), 3OHC18 (14%), 2 others (16%) C6 (51%), 3OHC10 (36%), 3OC4∶1 (14%) 0.31±0.08
BBD-FLK-1M27 3OC6 (29%), 3OC18∶1 (19%), C14 (19%), 3OHC10 (12%), 7 others (21%) 3OHC10 (100%) 1.19±0.12
1

Results are an average of four samples (two cultures sampled twice).

2

Results are an average of six samples (three cultures injected twice).

3

Percentages represent relative abundance within the culture.

4

AHLs are comprised of a homoserine lactone ring attached to an acyl side chain (generally between 4–20 carbons in length) and may have a keto or hydroxy substituent at the C3-position. Abbreviations: CX = AHL contains “X” carbon molecules in the acyl chain; 3OCX – AHL has a keto substituent at the C3-position; 3OHCX – AHL has a hydroxyl substituent at the C3-position.

5

Error is reported at standard deviation.

6

Not detected.

7

Isolate BBD-FLK-1M2 was identified through 16S rRNA gene sequencing as a 100% match to Ferrimonas sp. EF3B-B688 (Accession No. KC545309.1). This isolate tested negative in the Chromobacterium violaceum CV026 patch test and was not tested using the Agrobacterium tumefaciens NTL4(pZLR4) assay.