Figure 1. Expression of BASC (BRCA1-associated genome surveillance complex) related genes and p73 expression in response to Pac/²¹²Pb-trastuzumab.

Mice bearing i.p. LS-174T xenografts were treated by Pac/²¹²Pb- trastuzumab for 24 h. A. Expression of MSH2 and BRCA1 was determined by RT-PCR. Results represent the average of a minimum of three replications. B. Immunoblot analysis for MAD2 and CYCLIN B1 was performed with tumor collected 24 h after Pac/²¹²Pb-trastuzumab treatment. MAD2 and CYCLIN B1 were detected 22 kDa and 48 kDa, respectively. Equal protein loading control was GAPDH. C. Expression of p73, NOXA, and PUMA was determined by RT-PCR. Results represent the average of a minimum of three replications. D. Binding abundance to E2F1 was determined by ChIP using specific primers for p73 and BRCA1.