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. 2014 Sep 30;9(9):e108556. doi: 10.1371/journal.pone.0108556

Figure 2. Cellular responses of the combinations rLdHSP70 + rLdPDI/rLdHSP70 + rLdTPI/rLdHSP70 + rLdEL-2 in hamsters lymphocytes (lymph node cell).

Figure 2

(A)\ LTT response of mononuclear cells from normal, L. donovani infected (25 day p.c.) and treated hamsters in response to Con A, SLD and combinations (rLdHSP70 + rLdPDI/rLdHSP70 + rLdTPI/rLdHSP70 + rLdEL-2) at 10 µg/ml each. Proliferative response was represented as mean O.D of stimulated cells - mean O.D of unstimulated control cells. Each bar represents the pooled data (mean ± S.D. value) of 6 hamsters and the data represent the means of triplicate wells ± S.D. of each hamster as mean O.D of stimulated cells and mean O.D of unstimulated control cells. (B) Nitric oxide production (µM): Peritoneal macrophages were stimulated with the supernatants of stimulated lymphocytes (3 days for mitogen and 5 days for test antigens) of normal, infected and cured hamsters in response to combinations with rLdHSP70, SLD and LPS respectively at 10 µg/ml each. The estimation of NO production was done using Greiss reagent in supernatants collected from macrophage cultures 24 h after incubation and the absorbance of the reaction product was measured at 540 nm. Significance values indicate the difference between the SLD and combination with rLdHSP70 stimulation (*, p<0.05, **, p<0.01; ***, p<0.001).