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. 2014 Sep 30;9(9):e108556. doi: 10.1371/journal.pone.0108556

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© 2014 Jaiswal et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) IL-12, (B) IFN-γ, (C) IL-10, and (D) IL-4, in PBMCs from individuals of cured VL patients (n = 8) and endemic controls (n = 8) in response to recombinant proteins individually as well as their combinations with rLdHSP70 and SLD antigens, each data point represents one individual. The results were expressed as picograms (pg) of cytokine/ml, based on the standard curves of the respective cytokine provided in the kit. The lower detection limits for various cytokines were as follows: for 5.1 pg/ml for IFN- γ, 30.8 pg/ml for IL-12, 5.1 pg/ml for IL-4 and 6.9 pg/ml for IL-10. Values are given as concentration in pg/ml.