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. 2014 Sep 30;9(9):e108604. doi: 10.1371/journal.pone.0108604

Figure 8. qRT-PCR analysis of 10 cold tolerance candidate unigenes in C. heterophylla Fisch.

Figure 8

(A) Time course expression pattern of 10 cold tolerance candidate unigenes under 4°C cold stress (0 h, 2 h, 4 h, 8 h, 24 h) in C. heterophylla Fisch leaves. (B) Expression pattern of 10 cold tolerance candidate unigenes during four stages (NA, CA, MW, and DA) of overwintering in C. heterophylla Fisch floral buds. The gene names and the primers used for qRT-PCR analysis are shown in Table S4. Standard error of the mean for three biological replicates (nested with three technical replicates) is represented by the error bars.