Figure 7. AMP inhibits the αβ T cell proliferative response only in the presence of γδ T cells and the response is inhibited by the CD73 inhibitor APCP.

(A–E) Proliferative responses of CFSE-labeled αβ T cells isolated from immunized TCR-δ^-/- B6 mice (1×10⁵/well) assessed after in vitro incubation for 1 h with immunizing peptide and APCs alone (A) or in the presence of 10 µM AMP (B), 5% resting γδ T cells (C), AMP and γδ T cells (D), or AMP and γδ T cells and 3 µM APCP (E) in 96 well plate. The results shown are representative of those obtained in three separate experiments. (F–J) HPLC analysis showing the amount of adenosine in culture supernatants after 1 h culture of αβ T cells (3×10⁵/well) (F), γδ T cells (1×10⁵/well) (G), αβ T cells+1 mM AMP (H), γδ T cells+1 mM AMP (I), or γδ T cells+1 mM AMP+3 µM APCP (J) in 96 well plate. The adenosine peak is indicated by the arrow. Results shown are from a single experiment and are representative of three experiments.