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Two series of independent experiments were compared: (i) experiments 1 to 4 for proteins derived from HNEC cultures obtained from 4 CF NP patients vs. 4 NP patients (non CF); (ii) experiments 5 to 8 for proteins derived from HNEC cultures obtained from 4 NP patients (non CF) vs. 4 controls patients. Each couple of samples was matched randomly. For quantification of proteins, iTRAQ labeling coupled to LC-MS/MS was performed (see methods for details).
