Figure 5. Western blot analysis of ANXA1, STIP1, TERA, KPYM, HSPB1, EZRIN and 1433S in primary cultures of human nasal epithelial cells (HNEC) from NP (n = 4) and CFNP (n = 4).

A: Each protein was analyzed in three technical replicates. For normalization, the acrylamide gels were stained with Coomassie blue (total migrated protein detection) just after electro-transfer and scanned using the Odyssey LI-COR. Using the Odyssey software, protein intensity values were normalized with their corresponding loading control intensity values. B: Box plots of the intensity calculated with the Odyssey software, *p<0.01, Mann and Whitney test. (a.u.: arbitrary units). We report Maximum (max), minimum (min), first quartile (Q1), third quartile (Q3).