Figure 3. Expression of Nt-JAT2 in tobacco plants.
(A) Organ-specific expression of Nt-JAT2 mRNA in tobacco plants. Total RNA (7.5 µg) prepared from each tobacco organ was probed with 32P-labeled Nt-JAT2 fragment (0.5 kb) (top). The amount of total RNA applied to each lane is shown by EtBr-stained 18S rRNA (bottom). For comparison between NtJAT1 and NtJAT2 expression, analysis was performed using the same membrane as our previous study [15]. (B, C) Immunoblot analysis of Nt-JAT2 and Nt-JAT1 proteins in control (B) and MeJA treated (C) plants. Microsomes from tobacco leaves, stems and roots were subjected to electrophoresis, blotted, and incubated with antibody to Nt-JAT2 or Nt-JAT1. L, leaves (Leaves were numbered from top to bottom).