Figure 4. UHRF1 reduces the expression of KiSS1 by increasing the methylation of CpG nucleotides.

(A) The methylation analysis of the KiSS1 gene in 14 bladder cancer tissues and adjacent normal samples. BSQ analysis revealed that KiSS1 methylation frequency is markedly increased with bladder cancer samples. ** p<0.01. (B) A positive correlation is observed between KiSS1 methylation and UHRF1 expression (R² = 0.2091, p = 0.0036). (C and D) CpG island methylation status of KiSS1 was analyzed by bisulfite sequencing in RT4 cells. Nine individual clones were shown per cell line. CpG dinucleotides were represented as dark squares for methylated cytosines and open squares for unmethylated cytosines.