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. 2014 Sep 17;2014:265840. doi: 10.1155/2014/265840

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Copyright © 2014 Andrea Acebes-Huerta et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of lenalidomide on the proliferation of B cells. (a) PBMCs obtained from healthy donors (n = 10) and CLL patients (n = 17) were labeled with CFSE and cultured with 1 μM lenalidomide or DMSO for 14 days. CFSE fluorescence in B cells (gated as CD5⁺/CD19⁺) was analyzed by flow cytometry. The histograms show a representative CLL patient and a healthy donor. (b) The figure shows the compilation of the results obtained from healthy donors and CLL patients. Results are expressed as the fold induction of the percentage of proliferating B cells in lenalidomide-treated cells relative to the vehicle-treated control (**P < 0.01, Mann-Whitney U test).