Figure 5. Exposure to IL-6 enhances cytokine and chemokine production in SFMC.

SFMC were left to adhere on plastic for 3 hours in DMEM supplemented with 10% fetal calf serum (FCS). SFMC were pre-exposed to IL-6/sIL-6R in combination with IgG1 or with TCZ (50 µg/ml) for 1 hour. Cells were then left untreated (A) or stimulated with poly(I-C) and MDP (B), IL-1β (1ng/ml) (C). CXCL8, CCL2 and IL-1β levels were measured by ELISA. *p<0.05 for values from IL-6/sIL-6R-stimulated compared with NT cells.